Matching of Experimental and Virtual 2D Gel

  1. As a first step import a proteome

  2. Choose Tools->Matching Exp. and Vir. Gel:

    Menu Overlay
  3. Select your experimental gel image

    Note: Gel image must be in .png, .jpg or .gif format
  4. Select a protein that should be set as landmark from the list on the left side:

    Overlay
  5. Click on the corresponding part of your experimental gel to set the landmark:

    Set Landmarks
  6. Repeat step 5 for defining at least 5 landmarks equally distributed throughout your experimental gel

  7. Misplaced landmarks can be removed using the remove function:

    Remove Landmarks
  8. The correlation between your landmarks and the predicted pI / MW can be checked by choosing the Regression-tab and clicking on Show Regression

    Correlation
  9. In order to view the virtual 2D gel click the button Virtual Gel in the toolbar:

    Show Virtual Gel

    Experimental 2D gel overlayed with virtual one

  10. To identify proteins within a specific gel region choose the Identification icon in the toolbar:

    Identify Proteins
  11. Select the region of interest by dragging the mouse (moving while holding the left mouse button down) over the gel:

    Identify Proteins
  12. After releasing the mouse button you must enter a name for the selected protein group.

  13. All identified proteins are displayed in a new group:

    Protein Group