As a first step import a proteome
Choose Tools->Matching Exp. and Vir. Gel:
Select your experimental gel image
Select a protein that should be set as landmark from the list on the left side:
Click on the corresponding part of your experimental gel to set the landmark:
Repeat step 5 for defining at least 5 landmarks equally distributed throughout your experimental gel
Misplaced landmarks can be removed using the remove function:
The correlation between your landmarks and the predicted pI / MW can be checked by choosing the Regression-tab and clicking on Show Regression
In order to view the virtual 2D gel click the button Virtual Gel in the toolbar:
To identify proteins within a specific gel region choose the Identification icon in the toolbar:
Select the region of interest by dragging the mouse (moving while holding the left mouse button down) over the gel:
After releasing the mouse button you must enter a name for the selected protein group.
All identified proteins are displayed in a new group: